In-situ material characterization methods such as High Temperature Confocal Laser Scanning Microscopy and hot-stage Electron Back Scattered Diffraction allow direct observation of microstructural processes during simulated thermal treatment.In this paper V-bearing microalloyed low carbon steel with and without Mo addition is studied using a combination of in-situ methods.It is concluded that addition of 0.2 wt.%Mo into V-bearing steel restricts austenitic grain growth as well as limitation of formation of both Allotriomorphic and Widmanstatten ferrite through a coupled solute drag effect.