中华鳖是重要的名特优水产养殖动物,由于缺少商品化的细胞系,其病毒性疾病的防治研究难以深入开展；本研究旨在建立连续的中华鳖心肌成纤维细胞系。以中华鳖心肌组织为材料,采用半消化法对心肌组织进行消化,将心肌组织剪成1 mm3的组织块,之后转入25cm2细胞培养瓶,使之均匀铺满瓶底,添加2ml培养液倒置培养,3d之后补充培养液至10ml并翻转培养瓶正置培养。采用RPMI1640, MEM,DMEM 3种培
Human autosomal recessive primary microcephaly (MCPH) is a neural developmental disorder hallmarked by significantly reduced brain size and variable intellectual disability.Mutation of WD40-repeat pro
During mammalian evolution, surface areas of the cerebral cortex increase markedly leading to gyrus formation or folding to fit into a limited cranium.
Motor neurons (MNs) are unique because they project their axons outside of the central nervous system to innervate the peripheral muscles.Limb-innervating lateral motor column MNs (LMC-MNs) travel sub
Neuronal diversity, in large part, depends on the distinct morphology of dendrites that function as the receiving end of presynaptic axonal inputs or sensory stimulations.Neuronal dendrites often elab
Cell migration is an essential event of metazoan development.Understanding the dynamic behavior of cell migration in living animals is important but also challenging since the complicated extracellula
Intraflagellar transport (IFT), a bidirectional transport system between the ciliary base and tip, delivers ciliary precursors and turnover products for cilia assembly and maintenance as well as signa
Cilia/flagella are conserved eukaryotic organelles involving in cell motility, environment sensory and embryo development.Most of ciliary proteins are transported from the cell body by an elaborate tr
Motile cilia and eukaryotic flagella typically contain a9+2arrangement of axonemal microtubules attached with accessory structures, such as dynein arms and radial spokes, that are necessary for motili
Cilia are hair-like structures found on the surface of almost all polarized cells and defects on formation or function of cilia will led to a series of genetic disorders known as ciliopathies.