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Kidney transporters participate in the secretion and reabsorption of endogenous and exogenous chemicals.Many factors could influence their expression and affect drug disposition, efficacy and toxicity.This study was aimed to examine the development-and aging-associated variations in major renal transporters in rats including 6 uptake transporters : Oat1, Oat3, Oct1, Oct2, Oct3, and Oatp4c1 ,and 6 efflux transporters: Mdr1b, Bcrp, Mrp2, Mrp4, Mate1, and Mate2-K.The kidneys from SD rats during development (? 2,1,7,14,21 d) ,maturation (28,35,and 60 d) and aging (180,540 and 800 d) were collected and total RNAs were extracted,purified,and subjected to real-time PCR analysis.Total proteins were extracted for western-blot analysis.Results showed that Oat1 ,Oat3,Oct1 ,Bcrp,Mrp2,Mrp4 and Mate2-k was low in fetal kidneys and increased gradually after birth,and dramatically increased after maturation and adulthood (180 d) ,and maintained at that high level till 850 d of the age.Oct2, Oatp4c1 ,Mdr1b and Mate1 was low in fetal kidneys and increased gradually after birth, and increased dramatically after weanling, maturation and adulthood (180 d) ,but it was decreased at aging (850 d).Oct3 mRNA expression was low in fetal kidneys and newborn kidneys,and had two peaks at 35 d and 850 d.The selected protein expression showed the similar trend.Thus, kidney transporter expressions are affected by ontogeny and aging, which could impact drug disposition in children and elderly.