【摘 要】
：
The photonic crystal beads(PCBs)with structural color codes attract increasing attention in the field of multiplex bioassay.However,the complicated illumination light path of microscope and the spheri
【机 构】
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State Key Laboratory of Bioelectronics,Southeast University,Nanjing 210096,China;Lab of Environment
【出 处】
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2015年中国生物医学工程联合学术年会
论文部分内容阅读
The photonic crystal beads(PCBs)with structural color codes attract increasing attention in the field of multiplex bioassay.However,the complicated illumination light path of microscope and the spherical structure of PCBs introduce many deviations in the detection of codes and fluorescent information.To explore cause of deviations,four kinds of PCBs modified with FITC were observed under fluorescence microscope.Some fluorescence images of PCBs appeared dark spots or dark rings on the surface of PCBs and the fluorescent spectrums of these dark areas were modified,while others showed uniform fluorescent intensity and original fluorescent spectrums.The optical simulation and theoretical calculation were accomplished and the results showed that the directions of band-gap in different parts of PCBs were discrepant,which modify the spontaneous emission of fluorescent dyes.Our research has profound guiding significance for the detection of fluorescent intensity and the decoding process of PCBs in the field of multiplex bioassay.Besides,our theoretical analysis could also has a guide for application of other production based on photonic crystal which is sensitive to the angle of incident light.
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