The commonly used medium to cultivate recombinant Pichia patoris,BMGY and BMMY,are too costly to put into industrial practice.Thus the possibility of using low budget resources,such as the carbon sources: malt,wheat bran,corn,molasses starch and the nitrogen sources: fishmeal,wheat bran,soybean flour,urea,peanut powder as the basis of a culture medium for recombinant Pichia pastoris Bp17,was investigated in this study.