【摘 要】
：
As a naturally occurring polyphenolic acid,tannic acid,which is mainly present in grape and green tea,has strong antioxidant and anticarcinogenic activities.Pyruvate kinase isoenzyme M2(PKM2),as a key
【机 构】
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Institute of Biotechnology,Key Laboratory of Chemical Biology and Molecular Engineering of National
【出 处】
：
中国生物化学与分子生物学会2016年全国学术会议
论文部分内容阅读
As a naturally occurring polyphenolic acid,tannic acid,which is mainly present in grape and green tea,has strong antioxidant and anticarcinogenic activities.Pyruvate kinase isoenzyme M2(PKM2),as a key enzyme of aerobic glycolysis and malignant progression,plays an important regulatory role and is considered as the potential anticancer target.However,the inhibition effects and interaction between tannic acid and PKM2 is not well known.In this study,tannic acid showed anti-proliferative effects on HeLa and HepG2 cells.Tannic acid also has a satisfied inhibitory effect on pyruvate kinase activity of PKM2,with the IC50 values as 0.39 μM,but not the PKM2 expression.Furthermore,it was deduced that the existence of static quenching mechanism between tannic acid and PKM2.The values obtained for the thermodynamic parameter suggested that hydrophobic force played a major role in the interaction between tannic acid and PKM2.The results showed that tannic acid is worthy of consideration as potential inhibitor of PKM2.
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