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A thermoresponsive and cationic nanoparticles based on poly (N-isopropylacrylamide-co-((2-dimethylamino)ethylmethacrylate)) copolymers (poly (NIPA-co-DMAEMA)) was fabricated by the free radical polymerization.The diameter and zeta potential of the prepared nanoparticles was about 140 nm, and 13.02 mV at 25℃, respectively.The lower critical solution temperature (LCST) of the synthesized nanoparticles was about 41℃ and higher than the human body temperature.