In infertile couples, a male contribution to infertility is found in 45-50%.The cause of male factor infertility remains largely unexplained, but varicocele and genetic disorder are recognized as major causes leading to spermatogenesis disability.Genetic disorder leads to male infertility include chromosomal abnormalities and Y chromosome microdeletions.Chromosomal abnormalities (numerical or structural abnormalities) can be detected routine karyotype analysis.In non-obstructed azoospemia (NOA) patients, the incidence of chromosomal abnormalities is about 15%, and Europe Association of Urology (EAU) recommend the karyotype analysis in men with azoospermia or oligozoospermia (sperm concentration <10 million/mL).The most common chromosomal aberration causing male infertility is 47, XXY, Klinefelters syndrome (KFS).In genetic disorder, dose the extra X chromosome play a most important role? In NOA patients,its true.But, in patients with severe oligozoospermia, Y chromosome microdeletions are the most major causes but they cannot be detected by routine karyotyping.