论文部分内容阅读
Previously, we demonstrated that the crude saponin from codonopsis lanceolata has striking anti-mutagenic effects, and its ethanol extract inhibited the hepatic injury induced by alcohol.In the present study, we investigated the preventive effects of total saponin extracted from codonopsis lanceolata(CLS) on the hepatotoxicity induced by diethylnitrosamine(DEN).50 mice were randomly divided into five groups: normal group,DEN group, high dose of CLS group, middle dose of CLS group, low dose of CLS group.Mice of normal group were orally administrated with distilled water daily.DEN group and three dose of CLS groups were ip injected DEN 20mg/kg every other day.Simultaneously three dose CLS groups were orally administered at different dosage of CLS daily for 5 weeks.Then the mice were killed, the number of mitosis and Kupffer cells were counted by HE staining,the activity of glutathione S-transferases (GSTs) and content of malondialdehyde (MDA) and tumor necrosis factor alpha (TNF-α) in liver tissue were determined.The results showed that DEN treatment significantly (P<0.01) elevated the number of mitosis and Kupffer cells, MDA and TNF-α contents, reduced GSTs activities when compared to normal group.High dose and middle dose of CLS group showed significantly reduced mitosis and Kupffer cells, MDA and TNF-α contents, elevated GSTs activities when compared to DEN group (P<0.01 or P<0.05).Taken together, our data indicated that Codonopsis lanceolata ethanol extract is a potential chemopreventive agent on the hepatotoxicity induced by DEN.