Adeno-associated virus (AAV)-mediated gene transfer has been demonstrated to be an effective approach for treating Parkinsons disease (PD).Triptolide T10 is a monomeric compound isolated from tripterygium wilfordii Hook.f.(Thunder God vine), a traditional Chinese herb for anti-inflammatory medications.In the present study, we coadministered T10 with recombinant AAV2 in SH-SY5Y human neuroblastoma cells and in the striatum of C57BL/6 mice, and then evaluated the AAV-mediated gene expression levels.The results have shown that T10 significantly augmented the expression of AAV-mediated gene in a dose-dependent fashion without detectable cytotoxicity.As growing evidence indicated that inflammation contributed to the progression of PD, and the anti-inflammatory effect of T10 was shown in our previous studies, our data of T10 to enhance AAV transduction suggest that T10 might be potentially used as a facilitating reagent for the AAV gene therapy applications in neurodegenerative diseases.