Objective: To analyze the differentially expressed genes (DEGs) among hepatocellular carcinoma (HCC),para-cancerous tissue (PCT) and normal liver tissue (NLT) by using Agilent microarray and explore the target genes related to the development and progression of HCC.Methods: Total RNA of matched HCC,PCT and NLT of HCC patients was isolated using one step Trizol method and qualified using Lab-onchip method.cRNAs were synthesized,fluoresce labeled and purified after total RNAs purified.The RNAs of HCC and NLT,HCC and PCT were hybridized to Agilent microarray (21074 probes) respectively.