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目的从ABO基因水平探讨正常人群血型鉴定中出现天然抗体缺失的机制。方法2008年1月—2009年12月间收集的部分或全部缺失抗体导致ABO血型正反定型不一致的15个正常健康个体,运用吸收放散试验(检验红细胞表面血型抗原最为敏感的方法)未能检测出其抗原性。PCR扩增后直接序列分析AB
Objective To investigate the mechanism of natural antibody deletion in blood group identification of normal population from ABO gene level. Methods Fifteen normal healthy individuals, who had some or all of the missing antibodies collected between January 2008 and December 2009, resulting in inconsistent forward and reverse ABO genotypes, were not detected using the Absorption Dissociation Assay (the most sensitive method of testing erythrocyte surface blood group antigens) Its antigenicity. Direct Sequence Analysis After PCR Amplification