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To estimate the prevalence and public health significance of cryptosporidiosis in goats in China,1265 fecal samples from seven farms in Henan province and Chongqing city were examined for Cryptosporidium oocysts.The overall infection rate of Cryptosporidium was 3.48% (44/1256).Significant difference was observed among age groups,with the postweaned kids having the highest infection rate (4.58%; x 2=14.23,ρ < 0.01).Cryptosporidium spp.were characterized by PCR:restriction fragment length polymorphism (RFLP) analysis and DNA sequence analysis of the small subunit (SSU) rRNA gene.The SSU rRNA:based PCR identified three Cryptosporidium species,including C.ubiquitum (24/44) in Henan province and Chongqing city,and C.andersoni (16/44) and C.xiaoi (4/44) in Henan province.Cryptosporidium ubiquitum was found in all age groups,C.Andersoni in all age groups except for the postparturition nannies,and C.xiaoi in prE-weaned kids and pregnant nannies.Thus,these findings indicate goats are a potential source for zoonotic infections with the C.ubiquitum.More studies are needed for better understanding of differences in the transmission and public health significance of cryptosporidiosis in goats.