The aim of this study was to carry out a survey for the presence of Giardia duodenalis infection in canine using ELISA and PCR and to identify risk factors for infection.Samples from 318 dogs faeces living in nine cities in China were used in present study.Each sample was tested for the presence of G.duodenalis specific antigens using ELISA and 197 out of 318 samples were further examined for the presence of G.duodenalis using PCR.The overall rate of canines infected with giardiasis in present study was 16.04% and 15.22 % using ELISA and PCR,respectively.No significant difference was found between sex and Giardia positivity.Young dogs (up to 1 year) and living in communities were identified as risk factors for infection by multivariate logistic regression analysis.In conclusion, giardiasis in dogs was present in nine cities an China;as risk factors, young dogs (up to 1 year) and living in communities were of great significance.Giardia-infected canine should be treated for hygienic management to prevent transmission in dog-to-human.