Self-assembly of nanoparticles has attracted sustaining interest to build blocks spontaneously organize into ordered structures in the field of nanotechnology for the promising applications of nanoparticles [1,2].However,it is still a challenging process for organizing and controlling nanostructures with complicated and ingenious structure from the nanoscale to large scales with a narrow size distribution [3].Nanopore-based techniques,known as a prominent candidate for next-generation DNA sequencing,could be involved into the self-assembly system as a precisely template due to the controllable geometry and the extraordinary electrochemical properties [4,5].A concept of bipolar electrode impels the glass nanopipette to play a role in the combination of fabrication and nanopore techniques [6,7].Here,a nanopipette-based bipolar electrode was demonstrated to promote a self-assembly process of functionalized metal nanoparticle forming a cyclic two-demensional structure.This method provides a brand new strategy to regulate the dynamic processing and the interparticle distance with a remarkable reproducibility,which depicts an extensive application for intracellular imaging or specific therapy.