Objectives: To dissect the stable genetic mechanism of oil content in B.napus in different environments,and detect significant associated QTLs for oil content ratio by linkage analysis.Methods:The172numbersof recombinant inbred lines(RIL)population derived from a cross between male parent P174 and female parent GH06was cultivated in Chongqing,Beibei(2007-2008、2013-2014、2014-2015)、Germany,Giessen(2007-2008,2008-2009)、Germany,Hohenlieth(2008-2009).The high-density genetic linkage map consisting of 2795 SNP markersand covering1832.9 cM with an average marker interval of 0.66 cM was constructed by Liu et al.(2013).The oil content was measured by Near infrared spectrum instrument(Foss,NIR System 5000).The significant associated SNP loci were identified by Windows QTL Cartographer 2.5softwareusing composite interval mapping model.Results:A total of nine QTLs were identified on five different linkage groups,and a single QTL could explain 2.17%—10.94%of the phenotypic variation.Among them,qOCRA01 at A01 chromosome was repeatedly detected in Cq2015and Gi2009.Conclusions: The results indicate that the oil content is highly affected by environment.There are probably having regulatory genes for oil content sensitive to environment at the chromosome A01 and A06.Molecular markers closely linked with these QTLs could be also applied in marker-assisted selection of breeding of high-oil and stabilizing-oil Brassica napus.