Both Melittin(ME)and LL37(L)are small linear helical peptides with multiply effects including antibacterial,anti-inflammation,anti-cancer and so on [1-3].However,they also have the undesirable property of cytotoxicity to red blood cells at a lower concentration(2 μM and 25-30 mM)which largely limit application widely in clinic [1-3].Recently,hybridizing of different antimicrobial peptides(AMPs)has been a common practice for obtaining novel hybrid AMPs with elevated antibacterial activity but minimized cytotoxicity [4-5].The hybrid peptides ME(1-13)-L(17-30)(M-L)combining the hydrophobic N-teriminal fragment of melittin(M)with the core antibacterial fragment of LL37(L),was designed for the first time to explore its antibacterial activity and hemolytic activity against bacteria and sheep erythrocyte respectively.