Soybean isoflavone (SIF) has anti-aging properties and many other biological functions; however, SIF is difficult to reach higher blood concentration due to its rapid metabolism.Therefore, it is of great value to design and produce a sustained-release formulation that is able to maintain a stable level of plasma concentrations.In tiffs paper, soybean isoflavone sustained-release microsphere from chitosan and sodium alginate was prepared successfully.The important factors that determined the quality of the microspheres were the sodium alginate concentration in solution B, the ratio of soybean isoflavone to chitosan and the mixing speed.The relative yield,encapsulation efficiency and drug loading capability of SIF were much higher than the existing commercial formulations.In real gastrointestinal conditions, compared with the non-sustained release group, the release rate of SIF slowed down and the reaction time was prolonged.Animal experiments showed that sustained-release microspheres intensified the anti-aging potentials of SIF.Compared with the Non-sustained release (NSR) group mice, oral SIF/CHI microsphere treated mice were better in the Morris Water Maze Test (MWMT), the MDA level in the both plasma and brain of the sustained release(SR) group mice decreased, and SOD content was remarkably improved.