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The phosphate sensor in aqueous solution, 1, is synthetized by 4-amino-1, 8-naphthalimide, oxalyl chloride and methanol.It shows sensitive and selective response to PO43-in DMSO-HEPES (0.02M, PH=7.4, 7∶3 v/v) among multitudinous anions, with the colorless solution changing to jade green and fluorescence color changing from blue to green.