【摘 要】
:
目的:研究微波辅助提取赶黄草中槲皮素的最佳提取条件。方法:微波提取和水浴提取两种方法比较,采用紫外可见分光光度法,以芦丁作为标准对照,在510nm处测定槲皮素含量。结果:微波辅助提取赶黄草最佳提取条件为在255w、辐射20min、60%乙醇作溶剂固∶液=1∶10、粉碎度为40目、浸泡时间1h条件下产率最高。结论:采用微波辅助提取可以显著缩短提取时间、提高中药成分的提取率和提取质量,明显优于水浴提取
【机 构】
:
泸州医学院基础医学院,化学教研室,四川泸州,646000
论文部分内容阅读
目的:研究微波辅助提取赶黄草中槲皮素的最佳提取条件。方法:微波提取和水浴提取两种方法比较,采用紫外可见分光光度法,以芦丁作为标准对照,在510nm处测定槲皮素含量。结果:微波辅助提取赶黄草最佳提取条件为在255w、辐射20min、60%乙醇作溶剂固∶液=1∶10、粉碎度为40目、浸泡时间1h条件下产率最高。结论:采用微波辅助提取可以显著缩短提取时间、提高中药成分的提取率和提取质量,明显优于水浴提取法。
其他文献
烟酰胺腺嘌呤二核苷酸(NAD)是生物体内重要的辅酶,与其依赖的脱氢酶结合并行使催化功能时,通常以烟酰胺单核苷酸(NMN)朝向酶活性口袋内,腺嘌呤核苷酸(AMP)朝外的伸展状态形成复合物,反应过程经历结合、氢传递和解离过程.NAD与脱氢酶的结合力包括氢键、范德华力、疏水作用等,是其在生物体内参与众多生物化学反应的作用基础.
Most studies of drug discovery today are focused on developing small-molecule inhibitors and only small number of enzyme activators has been identified.However,finding allosteric activators is a promi
糖基化是最普遍却也最为复杂的一种蛋白质翻译后修饰.聚糖在信号传导、生物识别、胚胎发育等一系列生物过程中发挥着至关重要的功能.糖基化修饰必须得到严格的调控,其改变或失调往往伴随着各种人类疾病,如糖尿病、炎症和癌症等.
本研究合成了新颖的钛酸锶半导体纳米材料,用链霉亲和素将该纳米材料进行生物功能化,通过链酶亲和素对生物素化抗体的特异性识别,构建了一个高灵敏的新型AFP电化学免疫传感器.采用一步夹心温育后,然后加入酶的底物硫堇和过氧化氢,引发增强的电化学检测.
Many efforts have been devoted into developing novel materials for selective capture and enrichment of low-abundant phosphopeptides prior to mass spectrometic analysis.In the present study, octahedral
The protozoan parasite Trypanosoma brucei (T.brucei) causes human African trypanosomiasis (HAT) which is also known as sleeping sickness.It is a neglected fatal disease and continues to pose a major t
Phosphorothioation has been widely used in the development of small RNA drug.Recently DNA phosphorothioation has was reported as an endogenous occurrence in bacteria.It is a broadly interesting questi
以天然高分子壳聚寡糖为原料,通过在壳聚糖2位-NH2上引入部分疏水性烷基,在6位-OH上引入亲水性羟乙基,制得N-十二烷基-O,N-羟乙基壳聚糖(LGC)两亲性衍生物,分别用FTIR、1HNMR和元素分析等技术对其结构进行表征.此壳聚糖衍生物在水相中自组装形成胶束,以芘为荧光探针研究了其自聚集行为,结果表明制得的自聚体具有较小的临界胶束浓度(CMC为1.67×10-2mg/mL).
Elucidation of the molecular mechanisms of two-component signal transduction (TCST) system continues to be the most challenging target in molecular biology.
Spherical superparamagnetic iron oxide nanoparticles have been developed as T2-negative contrast agents for magnetic resonance imaging in clinical use because of their biocompatibility and ease of syn