Microbial Community Functional Diversity and Enzymatic Activity in the Sediments of Drinking Water R

来源 :2012水资源与城市化发展大会 | 被引量 : 0次 | 上传用户:weizai111
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  Sediment microbial communities act as an important role in the aquatic ecosystem by influencing nutrient cycling,organic matter metabolism,heavy metal transportation,and organic pollutant transformation.In this work,microbial community functional diversity and enzyme activity in sediments of two drinking water reservoirs were examined.Sediment bacterial and fungal communities functional diversity were determined using substrate utilization profiling (BIOLOG) method.Sucrase,dehydrogenase,urease,alkaline phosphatase activities were measured spectrophotometrically.The results shown that sucrase activity in Tang Yu (TY)reservoir was 329.77 mg glucose/g.24h,which is significant higher than that of Shi Bian Yu (SBY) (P<0.01).Dehydrogenase activities in SBY and TY reservoirs were 63.21 μg TF/h and 104.78 μg TF/h,respectively.However,there were no significant differences in the alkaline phosphatase activity between SBY and TY reservoirs (P>0.05).Average Well Color Development (A WCD590 nm) of bacterial community in sediment of TY was higher than that of SBY.The principle component analyses (PCA) revealed that PC1 and PC2 explained 35.92% and 22.63% of the total variation,respectively.There is a significant different carbon source utilization pattern of bacterial and fungal communities harbored in sediments of SBY and TY reservoirs,Northwest China.
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