Microorganisms such as pathogens,viruses,and parasites have been a serious threat to human health and the living environment.At present,most POCT detection based on microfluidic chips are optical detection devices.Not only are they costly to produce,but the lasers,photodetectors and cameras they need are not easily further miniaturized with sensors and integrated circuits(ICs).Thus fast,portable,inexpensive,and accurate biosensors to trace these microorganisms are in urgent need.Here we propose a nucleic acid detecting sensor combining electrochemical readout with Loop-Mediated Isothermal Amplification(LAMP)reactions[1].A new type of fabrication method would be used to make plastic threegold-electrode(PGE)with microfluidic system(Figure 1)[2],[3].This kind of chip is cheap,stable,and easily to be pattened and arrayed,well meeting the high requirement for portable detection.Figure 2 is a detection result gotten from a proof-of-concept gene target(HF183)on gold disk electrode.The kinetic SWV reading during HF183 LAMP reaction indicates that the signal could be measured in real-time function.The detection sensitivity can achieve as high as 100 copies per reaction,which meets the detection requirement for real samples.