In recent years, developing uncontaminated energy sources and alternative fuel have to become focal point in construction of energy sources for the future.Hydrogen is viewed as desirable clean energy in the future due to its clearing, high performance and non exhaustible.Among the various hydrogen production methods, biohydrogen production which was to utilize microorganisms is the important pathway.Compared with the traditional chemical process for the hydrogen production from fossil resources, biohydrogen production is promising for its capability of being renewable, clean and non-polluting.In this study, the hydrogen production yields have been improved, which not only could produce the clean energy sources, but also could deal with organic contaminant, meanwhile multifarious problem such as environment protection and resources reclaim will be solved by this technology.