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Background:Endothelialrecovery is a central feature of tissues after frostbite injuries.Thermotolerance plays an important role in protecting cellsagainst injury after frozen and thawing.The present study aimedto quantitatively assessthe injury of human umbilical vein endothelial cells HUVEC-12 after repeated low temperature.Material and method:Pretreatments (HUVEC-12) cells were repeatedly exposed to cold (1° C/ min decrement to-20° C).Their proliferation, death, apoptosis, and protein and mRNA expressions of HSP70 were determined.Results: Endothelial cells after repeated cold exposureswere more resistant toapoptosis and necrosis than normal cells.The expressions of HSP70 in cells after repeated cold exposures were significantly higher than in normal HUVEC-12 cells (P < 0.05).Conclusion: Cold acclimationmay induce the expression of HSP70 which plays a protective role in the temperature tolerance.