【摘 要】
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Objective This study was to investigate the role of TRPM7 in oxygen-glucose deprivation/reoxygenation (OGD/R)-induced apoptosis in human embryonic kidney cells (HEK293 cell line) and explore the possi
【机 构】
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Department of Neurobiology and Key Laboratory of Neurological Disease of Hubei Province, Tongji Medi
【出 处】
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中国神经科学学会第九届全国学术会议暨第五届会员代表大会
论文部分内容阅读
Objective This study was to investigate the role of TRPM7 in oxygen-glucose deprivation/reoxygenation (OGD/R)-induced apoptosis in human embryonic kidney cells (HEK293 cell line) and explore the possible implication of annexin-1 in this process.Methods Tetracycline-inducible HEK293 stable cell lines that expressed TRPM7 were created.Cell viability was determined by methylthiazol tetrazolium (MTT) assay.Apoptosis was quantitatively analyzed by flow cytometry using Annexin V-FITC and propidium iodide (PI) double staining.Results After 1 h OGD treatment, the viability of TRPM7-expressing cells was significantly lower than that of non-TRPM7-expressing cells during reoxygenation and reached the minimum at 24 h.Meanwhile, the apoptosis rate of TRPM7-expressing cells was significantly higher than that of non-TRPM7-expressing cells.However, after OGD/R following suppressing annexin-1 with antisense oligodeoxynueleotides (ASODN), above-mentioned phenomenons were reversed that the viability of TRPM7-expressing positive cells was markedly increased and the apoptosis rate was significantly reduced.At equal, Annexin-1 protein expression was gradually increased and translocated from cytoplasm to cell membrane and preferentially to nucleus after OGD/R in TRPM7-expressing cells.Further studies revealed that TRPM7 and annexin-1 were co-localization in TRPM7-expressing cells, TRPM7 interaction with annexin-1 was visibly enhanced by OGD/R treatment in a kinase-dependent and Ca2+-independent manner.Conclusion The results indicated that TRPM7 participated in OGD/R-induced apoptosis in HEK293 cells and the annexin-1 was involved in apoptosis process which afforded by TRPM7.
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