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  5. Single pollen nucleus sequencing reveals chromosome fragmentation as a possible cause of haploid ind

Single pollen nucleus sequencing reveals chromosome fragmentation as a possible cause of haploid ind

来源 :第七届全国生物信息学与系统生物学学术大会 | 被引量 : 0次 | 上传用户：A312685521
【摘 要】
：
Production of maternal haploids via intra-specific genotypes as the haploid inducer is routine and highly efficient in maize.However,the underlying mechanism of haploid induction (HI) is unclear.Triph
【作 者】
：
Xiang Li Dexuan Meng Shaojiang Chen Haishan Luo Qinghua Zhang Weiwei Jin Jianbing Yan 
【机 构】
：
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University,Wuhan 430070,
【出 处】
：
第七届全国生物信息学与系统生物学学术大会
【发表日期】
：
2016年8期
【关键词】
：
Single Cell Genomics Haploid Induction Copy Number Variation Chromosome Fragment 
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　　Production of maternal haploids via intra-specific genotypes as the haploid inducer is routine and highly efficient in maize.However,the underlying mechanism of haploid induction (HI) is unclear.Triphenyltetrazolium chloride (TTC) staining data indicated that the viability,rather than fertility,of the inducers mature pollen is lower than that of regular lines.We have developed a method to isolate the three nuclei from a mature pollen grain and four microspores from a tetrad for whole-genome sequencing.
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