【摘 要】
：
Microfluidic chip-based liquid-chromatography coupled to mass spectrometry (LC-MS) has played an important role in proteomic research for high throughput analysis.Up to date, the use of chip-based LC-
【机 构】
：
Department of Chemistry, Soochow University, Taipei, Taiwan
【出 处】
：
第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
论文部分内容阅读
Microfluidic chip-based liquid-chromatography coupled to mass spectrometry (LC-MS) has played an important role in proteomic research for high throughput analysis.Up to date, the use of chip-based LC-MS for quantitative determination of small molecules, such as active pharmaceutical ingredients (APIs) and abused drugs/drug metabolites, in pharmaceutical and clinical applications has not been thoroughly investigated.This paper will discuss the utilization of chip-based LC-MS systems for quantitative determination of small molecules (including abused drugs/drug metabolites and disease/tumor-associated biomarkers) for bioanalytical application.A integrated chip-nanoLC device integrates a microfluidic switch, a chip column design having a pre-column (enrichment column) for sample enrichment prior to an analytical column for separation, as well as a nanospray emitter on a single polyimide chip was utilized in this study.The approach to minimize ion suppression of urine sample will be presented; furthermore, the stability and lifetime of chip will be evaluated.In addition, the applicability of microfluidic chip-based LC-MS for the analysis of aflatoxins in food matrix will also be demonstrated.
其他文献
An estimate of approximately one million dollars per year are spent by a large company on purchasing organic solvents required for running reversed-phase liquid chromatography (RPLC) and waste disposa
Temperature has been considered as an important variable in Liquid Chromatography (LC).Operating at higher temperatures has led to "High Temperature Liquid Chromatography" which has attracted major at
Although supercritical fluid chromatography was demonstrated in 1962, its applications had been quite narrowly limited due to the difficulties on SFC instruments availability for near 40 years, which
In this work, trypsin immobilized onto a macroporous polymer monolith in a pipette tip for digesting proteins has been developed to explore the utility of using a tip-based protein digestion methodolo
Ion suppression has been demonstrated to have a significant effect on the quantitation of a range of molecules, particularly in the presence of a complicated matrix.One area where this has been preval
It is necessary to determine the macromolecule impurities content in β-lactam antibiotics for quality control.In China, the quality control method is usually performed by low-pressure gel filtration c
A 3D bicontinuous skeleton macroporous monolith was firstly prepared by in situ atom transfer radical polymerization (ATRP) of ethylene glycol dimethacrylate (EDMA) at the room temperature.The structu
Melamine (1,3,5-triazine-2,4,6-triamine), a kind of triazine analogue with three amino groups, is an industrial chemical used in the production of melamine-formaldehyde resins.Because of the substanti
Especially in the field of proteomics research, fast and efficient miniaturized separation methods are required to analyze very small amounts of sample.As a result, there is a strong interest in the d