【摘 要】
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Objective: To develop a procedure for determining the DNA methylation status of the SHP-1 gene in gastric cancer.We established a new bisulfite-based methylation-specific polymerase chain reaction (MS
【机 构】
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School of Biomedical Engineering,Med-X Research Institute,Shanghai Jiao Tong University,Shanghai,Chi
【出 处】
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22nd Asia Pacific Cancer Conference(第22届亚太抗癌大会)
论文部分内容阅读
Objective: To develop a procedure for determining the DNA methylation status of the SHP-1 gene in gastric cancer.We established a new bisulfite-based methylation-specific polymerase chain reaction (MSP) method and applied the technique to determine the DNA methylation status of theSHP-1 promoter region of MKN45 gastric cancer cells.We further explored the potential of the methylated SHP-1 gene as a marker for early cancer detection and prognosis prediction, as well as the application of demethylation drugs in gastric cancer therapy.
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