Lymphatic-targeted vaccine delivery has been reported as a potential strategy to enhance the immunogenicity of vaccines.However,it remains unclear whether enhancing the lymphatic targeting of liposome vaccines could promote immunological memory,a major determinant of vaccine efficacy.In the present study,DOTAP liposomes (LP) were incorporated with mannosylated DSPE-PEG (PEGMan) to acquire mannosylated liposomes (LP-Man) (Fig.1).The result of in-vivo fluorescent imaging showed that non-targeted LP mostly accumulated at the vaccination site,confirming its depot effect.