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In the post-genome sequencing era, proteomics study becomes more and more important.Compared to genomics, the analysis of proteome is rather difficult.To meet the higher and higher requirements from proteome analysis, it is urgent to develop novel high efficient separation and high sensitive detection techniques.The target of our recent research is to establish a high resolution separation, high sensitive detection and high accuracy identification platform for proteome study, including on-line concentration, two-dimensional separation, on-line digestion and MS detection.