目的 The aim of the investigation was to target analysis the predominant characteristics of ESBL-producing Klebsiella pneumonia(K.pneumoniae)strains from patients with lower respiratory tract infection in Guizhou province.方法 From January 2015 to February 2016,69 of 237 K.pneumonia strains producing ESBL from patients with lower respiratorytract infection were collected from four tertiary hospitals from Guizhou province.Phenotypic methods and antibioticsusceptibility tests were employed to characterize 69 ESBL-producers.The genotype of ESBL were analysed by amplification ofPCR,direct sequencing with various set of PCR primers and the basic local alignment search tool online.Sample cluster analysisof the detection results was also performed.