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In order to assess the effects of the surface hydrophilicity of supports on the biofilrn formation and evaluate the performance of completely autotrophic nitrogen removal over nitrite (CANON) process in a sequencing batch biofilm reactor (SBBR), unmodifed activity carbon fibers (ACFs) and ACFs hydrophilic-modified by heat treatment were used as supports.CANON process was initiated in a SBBR from conventional activated sludge.