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Field microcosm experiments,lab incubation,15N labeling techniques,PCR-DGGE (denaturing gradient gel electrophoresis),and quantitative polymerase chain reaction (QPCR) were used to investigate the effects of biochar addition on rice N nutrition and GHG emissions in an Inceptisol and an Ultisol,and on bioavailability of persistent organic pollutant (POP).