目的 To investigate the in vitro regulatory mechanism of ER-β of OPN-Integrin interaction in the migration of human lung cancercells.方法 The expression of ER-βas well as OPN and Inregrin in human lung cancer cell lines was investigated.The expression changeand cell membrane distribution of OPN and Inregrin after E2 stimulation was explored using using RT-PCR,Western blot andflow cyrometry.The impact of E2 stimulation,OPN siRNA inteferecnce and Integrin neutrolizing antibody on the migratorycapability of lung cancer cells was evaluated using Transwell and CIQ Alive Image Monitoring System.The potential signalingpathway relevant to E2-induced Integrin cell membrane distribution was explored,as well.