【摘 要】
：
目的：通过分析钙网织蛋白(CRT)在类风湿关节炎(RA)患者体内的表达水平,进一步探讨CRT在RA发病机制中的作用.方法：纳入本研究的血清标本：活动期RA 44例、骨性关节炎(OA)16例、系统性红斑狼疮(SLE) 25例、其他自身免疫性疾病24例及健康对照(HC) 30例,同时选取7例RA、14例OA滑液及滑膜标本.采用ELISA检测血清及滑液中CRT的含量,Western blot分析血清中C
【机 构】
：
天津医科大学总医院感染免疫科 天津医科大学医学检验学院
论文部分内容阅读
目的：通过分析钙网织蛋白(CRT)在类风湿关节炎(RA)患者体内的表达水平,进一步探讨CRT在RA发病机制中的作用.方法：纳入本研究的血清标本：活动期RA 44例、骨性关节炎(OA)16例、系统性红斑狼疮(SLE) 25例、其他自身免疫性疾病24例及健康对照(HC) 30例,同时选取7例RA、14例OA滑液及滑膜标本.采用ELISA检测血清及滑液中CRT的含量,Western blot分析血清中CRT的存在形式,病理学方法检测CRT在RA和OA关节滑膜组织中的表达.结果：RA组血清中CRT含量[(4.817±2.423) ng/ml]高于OA组[(3.574±0.923) ng/ml]、SLE组[(4.013±1.524) ng/ml]、其他自身免疫性疾病组[(3.882±0.842) ng/ml]以及健康对照组[(3.726±0.619) ng/ml],差异有统计学意义,p＜0.05.RA组与OA组滑液中CRT含量无统计学差异.CRT在血清中仅存在单体一种形式.RA滑膜组织中可见大量炎性细胞浸润,CRT呈现高表达,主要位于滑膜组织的衬里层和衬里下层、血管内皮细胞以及血管周围；在OA,CRT仅在衬里层及血管周围有极少量的表达.结论：RA血清中CRT含量明显增高,在滑膜组织中呈高表达,且表达位置不同于OA.提示CRT可能参与了RA的关节炎症反应及血管翳的形成,有望成为诊断RA的潜在血清学指标.
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