【摘 要】
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Antigen cross-presentation in dendritic cells is a complex intracellular membrane transport process,but the underlying molecular mechanisms remain to be thoroughly investigated.In this study,we examin
【机 构】
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Institute of Immunology, PLA, Third Military Medical University, Chongqing 400038, China
【出 处】
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全军免疫学第七届学术交流会暨免疫学新进展主题论坛
论文部分内容阅读
Antigen cross-presentation in dendritic cells is a complex intracellular membrane transport process,but the underlying molecular mechanisms remain to be thoroughly investigated.In this study,we examined the effect of siRNA-mediated knockdown of 57 Rab GTPases,the key regulators of membrane trafficking,on antigen cross-presentation.Twelve Rab GTPases were identified to be associated with antigen cross-presentation,and Rab3b/3c was indicated to be co-localized with MHC class Ⅰ molecules at perinuclear tubular structure.Tracing with fluorescence protein tagged β2-microglobulin demonstrated that the MHC class Ⅰ molecules were intemalized from the plasma membrane to Rab3b-and Rab3c-positive compartments,which were also co-localized with the internalized transferrin.Moreover,depletion of Rab3b/3c strongly reduced the fast phase recycling rate of transferrin receptors.
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