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Cytosine methylation is a well recognized epigenetic mark.Here, the methylation status of a salinity tolerant (cv.SR3, derived from a somatic hybridization event) and its progenitor parent (cv.JN177) wheat cultivar was explored both globally and within a set of 24 genes responsive to salinity stress.A further comparison was made between DNA extracted from plants grown under control and when challenged by salinity stress.