【摘 要】
：
In eukaryotes, aberrant expression of transposable elements (TEs) is detrimental to the host genome.Piwi-interacting RNAs of~23 to 30 nucleotides (nt) bound to PIWI-clade Argonaute proteins silence tr
【机 构】
：
Interdisciplinary Research Center on Biology and Chemistry,Chinese Academy of Sciences,Shanghai,Chin
【出 处】
：
2015 Genomics Frontiers Symposium(2015基因组学前沿研讨会)
论文部分内容阅读
In eukaryotes, aberrant expression of transposable elements (TEs) is detrimental to the host genome.Piwi-interacting RNAs of~23 to 30 nucleotides (nt) bound to PIWI-clade Argonaute proteins silence transposons strictly dependent on their sequence complementarity.Hence, a key question in understanding piRNA pathways is to determine mechanisms that modulate piRNA sequences.Here,we identify a protein-protein interaction between Nibbler (Nbr), a 3-to-5 exoribonuclease, and Piwi,linking Nbr activity with piRNA pathways.We show a delicate interplay between Nbr and Hen 1, a methyltransferase involved in 2-O-methylation at 3 terminal nucleotides of piRNAs, connecting two genes with opposing activities in biogenesis of piRNA 3 ends.With age, piRNAs become shorter and fewer, coupled with de-repression of selected TEs.We demonstrate that activities of Nbr and Hen1 inherently contribute to TE silencing and age-dependent profiles of piRNAs.We propose that antagonistic roles between Nbr and Hen1 define a mechanism to modulate piRNA 3ends.
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