Carbon nanoparticles (CNPs) (8-15nm in size) were prepared by refluxing corn stalk soot in nitric acid.The obtained acid-oxidized CNPs are soluble in water due to the existence of carboxylic and hydroxyl groups.C13 NMR measurement shows the CNPs are mainly of sP and sp3 carbon structure.Furthermore, these CNPs also exhibit unique photoluminescence properties.Interestingly, the CNPs might be exploited to immobilizate on the surface of porous silica particles by means of intermolecular polycondensation as chromatography stationary phase.The resultant packing material was evaluated by high-performance liquid chromatography, indicating that the new stationary phase could be used in hydrophilic interaction liquid chromatography (HILIC) and per aqueous liquid chromatography (PALC) modes.The separation of five nucleosides, four sulfa compounds and safflower injection were achieved by using the new column in the HILIC and PALC modes, respectively.