Experimental Study on Construction of Tissue Engineering Adipose With Human Umbilical Cord Mesenchym

来源 :第三届全球华裔整形外科医师大会 | 被引量 : 0次 | 上传用户:frankcomet
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Objective To study the construction of tissue engineering adipose in vitro with silk fibroin scaffold and human umbilical cord mesenchymal stem cells (hUCMSCs)transfected by recombinant human insulin gene lentivirus.Methods 1.hUCMSCs infected with recombinant lentiviral pLenti6(.)3-insulin-IRES-EGFP(Group A) by the best MOI (MOI=10)were seeded in silk fibroin scaffold;hUCMSCs transfected by EGFP gene (Group B)was regarded as negative control;and then the cell-scaffold complexes were cultured in adipogenic differentiation medium.2.
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