【摘 要】
：
LC-MS is one of the most widely used analytical platforms in metabolomics.However, the highly diverse metabolites in biological samples exhibit distinct chromatographic behavior and require the combin
【机 构】
：
State Key Laboratory of Quality Research of Chinese Medicine, Institute of Chinese Medical Sciences,
【出 处】
：
2015年第一届药代动力学朝阳论坛
论文部分内容阅读
LC-MS is one of the most widely used analytical platforms in metabolomics.However, the highly diverse metabolites in biological samples exhibit distinct chromatographic behavior and require the combined use of reversed-phase (RPLC) and hydrophilic interaction chromatography (HILIC) to increase metabolome coverage.This is usually done in two separate runs due to the solvent strength incompatibility between RPLC and HILIC, inevitably increasing the analysis time, sample consumption and redundant data.For untargeted metabolites identification, MS2 spectra are usually collected through data-dependent acquisition (DDA).When dealing with complex metabolome ocean, top n based DDA is just scratching the surface as only a small fraction of the ions could be selected for MS2.Here, we developed a direct-coupled RPLC-HILIC for metabolomics which affords good chromatographic behaviors for a variety of endogenous hydrophilic and hydrophobic metabolites with variation of retention time and peak area below 2% and 20%, respectively, making the global metabolome coverage possible in a single run.Furthermore, we developed an improved DDA method for untargeted metabolomics using gas-phase fractionation with staggered mass range (sGPF).
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