液相色谱-高分辨质谱法快速鉴别赤潮藻中的麻痹性贝毒素

来源 :第十一届中国生物毒素研究及医药应用年会 | 被引量 : 0次 | 上传用户：huier0001

【摘要】

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【作者】

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李兆永陈军辉张茹潭王艳龙史倩王帅郑立王小如

【机构】

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国家海洋局第一海洋研究所,海洋生态研究中心,山东青岛 266061 国家海洋局第一海洋研究所,海

【出处】

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第十一届中国生物毒素研究及医药应用年会

【发表日期】

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2013年11期

【关键词】

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赤潮麻痹性贝毒素亲水作用色谱高分辨质谱筛选

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　　本研究采用亲水作用色谱-高分辨电喷雾质谱联用技术(HILIC-HR-ESUMS)结合麻痹性贝毒素(PSP)精确质量数数据库，建立快速筛查、定性识别赤潮藻中常见PSP的方法.产毒藻细胞破碎后经0.1 mol/L乙酸水溶液提取，通过HILIC-HR-ESI/MS正负离子模式全扫描分析，得到产毒藻粗提物中PSP化合物准分子离子及主要碎片离子精确质量数，结合PSP数据库筛查并辅以商品化软件数据分析，实现产毒藻所含PSP的快速定性识别.所建数据库包含25种常见PSP化合物精确分子质量及碎片离子信息，发展的HILIC-HR-ESI/MS方法分离度高、灵敏度好，所考察的10种常见PSP化合物检测限在10-80 nM范围内，能满足产毒藻实际样品的分析要求.3种产毒藻实际样品筛查、分析结果良好，说明该方法是赤潮藻中麻痹性贝毒素快速筛选、定性识别的有效方法.

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液相色谱-高分辨质谱法快速鉴别赤潮藻中的麻痹性贝毒素

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