In this study,we attempted to integrate a gene expression frame composed of a constitutive promoter P43,functional gene sfp,and pleiotropic regulatory gene degQ into the chromosomal amyE locus of the
脂肪氧合酶(LOX)是一种含非血红素铁的蛋白,催化具有顺,顺-1,4-戊二烯结构的多元不饱和脂肪酸的双加氧反应,如亚油酸、亚麻酸。脂肪氧合酶广泛存在于植物、动物中,在微生物中鲜有发现。尽管人们早已认识到LOX在食品工业、医药工业和化工领域中的潜在应用价值,但从自然界中直接获得不仅得率低而且纯化难,获得LOX纯酶的成本很高。通过LOX基因的克隆与表达,经简单的纯化过程,获得高纯度的LOX酶是解决上述
研究背景：抗菌脂肽是由革兰氏阳性芽孢杆菌通过非核糖体合成途径产生的具有抗菌作用的脂肽类化合物，是天然食品防腐剂的一种，在抗植物真菌感染以及对抗某些疾病方面具有潜在应用价值。酵母菌在低湿度、低pH值或高盐高糖的食品基质中具有较强的适应能力，容易引起食品腐败。为了有效控制这类食品中酵母菌污染，有必要开发一种安全，高效、对酵母菌有抑制作用的抗菌脂肽。
Wheat,a staple food,is the major nutritional components for both food and feed and represents the key food of humankind.However,wheat is commonly colonized by Fusarium species from the field.Fusarium
Chitin is one of the primary structural components of the fungal cell wall.Chitinases are suggested to play a role in disintegration and liquefaction of the mature pileus of basidiomycete coprinoid mu
银杏是银杏科(Ginkgoaceae)银杏属(Ginkgo)的多年生落叶乔木，是现存裸子植物中最古老的孑遗植物。其叶和果均可入药，银杏叶提取物(GBE)已经被研制成多种制剂，对心脑血管疾病有较好的疗效。有研究发现从银杏内生菌的次生代谢产物中可以分离出与银杏叶提取物类似的物质，因此，研究银杏叶内生菌多样性可以为进一步探索银杏内生菌功能多样性提供理论基础。
A putative class Ⅲ endochitinase (ChiⅢ) was reported previously be expressed dominantly in fruiting bodies of Coprinopsis cinerea and its expression levels increased with the maturation of the fruitin
A gene coding endo-β-1,3(4)-glucanase(ENG16A)was cloned from Coprinopsis cinerea and heterologously expressed in Pichia pastoris.ENG16A only acts on substrates containingβ-1,3 glycosidic bonds but not
植物体内普遍存在着内生菌，其中很多内生菌具有天然生物活性，包括抗菌、抗肿瘤、杀虫、免疫抑制和抗氧化等作用。内生菌在植物体内生存稳定、又不引起植物明显病害，不受环境条件影响且可与环境保持友好关系，无论对医学应用、农业发展还是环境保护问题都具有不可估量的价值。近年来，植物内生菌作为一种新型微生物资源引起了学术界的广泛关注。
53 bacteria were isolated from Pinellia ternate and 23 of them were identified by 16S rRNA sequencing.The isolates in this study can be classified into 7 genera with Bacillus as the predominant genus.