To investigate the embryo quality and pregnancy outcome of patients with a high risk of fertilization failure who were treated with selective, short-term fertilization and early rescue ICSI(Intracytoplasmic Sperm Injection).This is a retrospective analysis of 2023 ART (Assisted Reproduction Technology) patients were grouped into 4 groups (short-term IVF, regular IVF, short-term ICSI, regular ICSI).In this study found that when compared with the regular IVF group, the fertilization rate, 2 PN rate and 1 PN rate were significantly lower in the short-term IVF group with rates of 70.9% vs 80.8%, 44.8% VS 60.9% and 3.5% VS 6.2%, respectively.However, the 0 PN rate and 3 PN rate were significantly higher than those in the regular IVF group, with 13.0% VS 5.4 and 9.6% VS 8.3%, respectively.Of note, the rate of available embryos in the short-term IVF group was significantly higher than for the regular IVF group (55.3% VS 52.3%).No significant differences were observed in the clinical pregnancy rate, miscarriage rate, ectopic pregnancy rate, live-birth delivery rate and birth defect rate between the short-term and regular IVF groups.The fertilization rates in the short-term ICSI and regular ICSI groups were not significantly different (77.9% VS 80.8%), which was also true for the cleavage and available embryo rates, miscarriage rate, ectopic pregnancy rate, live-birth delivery rate and birth defect rate.The multiple PN rate in the short-term ICSI group was significantly higher than that of the regular ICSI group (7.0% VS 1.9%).The clinical pregnancy rate in the short-term ICSI group was higher than that of the regular ICSI group (51.2% VS 42.3%), but this difference was not significant.Based on these results ,selective, short-term fertilization can result in effective outcomes for patients with a high risk of fertilization failure.