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A method of determining of five phenolic compounds in human urine with Magnetic Extraction and High Performance Liquid Chromatography(HPLC)was established by using Agilent1260 liquid chromatography—ultraviolet detector and C18(250 × 4.6 mm id,5.0μm)column.Chromatographic conditions were as follows: mobile phase: methanol: water = 55: 45(v: v); column temperature: 30 ℃;wavelength : 270nm; injection volume: 10μL; flow rate: 0.8μL/min.