【摘 要】
：
目的:本文建立了液相色谱-串联质谱(LC/MS/MS)法同时测定大鼠血浆中的5种蟾蜍甾二烯类浓度的方法.方法:色谱柱:ZORBAX SB-C18 (3.5μm,2.1mm×100 mm)和C18保护柱;流动相:乙腈-水-甲酸(50∶50∶0.05,V/V/V);流速:0.3ml/min.结果:5种蟾蜍甾二烯类化合物的最低定量限均为1ng/ml,检测方法的线性良好,其相关系数均大于0.995,日内精
【机 构】
：
四川省中医药科学院分析测试中心,四川成都610041;第二军医大学天然药化教研室,上海200433
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目的:本文建立了液相色谱-串联质谱(LC/MS/MS)法同时测定大鼠血浆中的5种蟾蜍甾二烯类浓度的方法.方法:色谱柱:ZORBAX SB-C18 (3.5μm,2.1mm×100 mm)和C18保护柱;流动相:乙腈-水-甲酸(50∶50∶0.05,V/V/V);流速:0.3ml/min.结果:5种蟾蜍甾二烯类化合物的最低定量限均为1ng/ml,检测方法的线性良好,其相关系数均大于0.995,日内精密度和日间精密度均符合要求.结论:本方法具有良好的灵敏度,准确度、精密度和专属性,结果准确,重现性好,易于操作,可用于蟾酥类制剂的大鼠体内药代动力学研究.
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