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Chinese herbal medicines have been used in anticancer therapy.The purpose of this study is to verify the anticancer of Shi-Ni San(四逆散),Sha-Su San(香苏散),Chi-Hu-Su-Gan Tan(柴胡舒肝汤),Sho-Saiko-to(小柴胡汤),Dan-Gua-Shau-Yiua Tan(当归芍药汤),Huang-Lian-Gia-Do Tan(黄莲解毒汤)、Sha-Fu-Ju-Yu Tan(血府逐瘀汤)、Ban-Sha-Ho-Po Tan(半夏厚朴汤)、San-Jou-Qai-Gian Tan(散肿溃坚汤)、Zi-Gan-Mo-Li Tan(紫根牡砺汤)in liver cancer cells in vitro.1,1-dipheny-2-picrylhydrazyl (DPPH) scaveng-ing activities of the water extracts of the 10 kinds of Chinese herbal medicines are analyzed by spectrophotometry.The concentrations required for 50% inhibition (IC50) of DPPH radicals were determined,and the results indicated that the herbal phenolic content showed a positive correlation with the DPPH scavenging capacity of each Chinese herb.Among them,Sho-Saiko-to(小柴胡汤),Shi-Ni San (四逆散),and Sha-Fu-Ju-Yu Tan(血府逐瘀汤)showed a lower 50% inhibition(IC50) by MTT analysis.