An ultra-high-performance liquid chromatography-triple quadrupole mass method in dynamic multiple reaction monitoring mode was developed and validated to simultaneously quantify 5 compounds in rat plasma after oral administration of safflower powder.These compounds are hydroxysafflor yellow A (1), bidenoside C (2),6-hydroxykaempferol-3,6-diglucoside (3), chlorogenic acid (4) and p-coumaric acid (5).Plasma protein was precipitated with methanol-ethanol (1:1).Chromatographic separation was carried out on a Zorbax Eclipse Plus C18 column (2.1 ×50 mm) using 0.1% formic acid water-methanol as mobile phase.Analysis of the standard curves showed good linearity (r > 0.996) and the RSD of intra-and inter-day measurements were both less than 11.3 % with accuracies ranged from-8.0 %to 9.4 %, and recoveries of plasma sample from 86.3% to 107.5%.Among the 5 quantified compounds, bidenoside Cdisplayed the largest AUCo-t value (2177.796 ng.h/L), followed by hydroxysafflor yellow A (1564.1 ± 277.4 ng·h/L).The pharmacokinetic behavior of bidenoside C is reported here in for the first time and its favorable pharmacokinetic parameters indicated that bidenoside C, in addition to hydroxysafflor yellow A, could play important role in the in vivo pharmacologic effects of safflower.