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5. Prokaryotic Expression and Purification of the soybean 7s protein α′-subunit

Prokaryotic Expression and Purification of the soybean 7s protein α′-subunit

来源 :第七届国际大豆加工利用会议 | 被引量 : 0次 | 上传用户：houjhz

【摘 要】

：

To obtain the N-linked glycans lacked α′ subunit of soybean 7S protein,the total RNA were extracted from the seeds of soybean variety Lu 96150.The α′ subunit coding sequence were amplified by RT-PCR o

【作 者】

：

Yanyan Xu Yong Chen Yujing Zhang Eizo Tatsumi Guangzhong Luan 

【机 构】

：

College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China

【出 处】

：

第七届国际大豆加工利用会议

【发表日期】

：

2016年4期

【关键词】

：

recombinant protein β-conglycinin cloning soybean subunit 

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　　To obtain the N-linked glycans lacked α′ subunit of soybean 7S protein,the total RNA were extracted from the seeds of soybean variety Lu 96150.The α′ subunit coding sequence were amplified by RT-PCR one step assay,and then inserted into the vector of pGEM-T Easy to construct the recombinant cloning vectors pGEM-α′.

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