3D organotypic epithelial structures can be engineered using primary or immortalised oral keratinocytes.However, their detailed quantitative histological characterisation in comparison with normal mucosal architecture has not been performed until now.Recent advances in quantitative microscopy now enable reproducible and quantitative morphological tissue characterisation.To identify morphological markers of tissue arrangement that can be used to monitor tissue structure, maturation and differentiation and enable quantitative comparison of organotypic cultures with normal oral mucosa.